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genome wide crispra screen  (Addgene inc)


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    Addgene inc genome wide crispra screen
    Genome Wide Crispra Screen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genome wide crispra screen/product/Addgene inc
    Average 94 stars, based on 25 article reviews
    genome wide crispra screen - by Bioz Stars, 2026-05
    94/100 stars

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    Fig. 1 | CRISPR activation screen and transcriptome analysis of patient samples identify PRMT3 as a candidate driver for OXA resistance in HCC. a Volcano plot reveals differential gRNAs targeting genes of genome-wide CRISPRa screen during OXA treatment. b Volcano plot shows the differentially expressed genes identified from RNA-seq analysis of HCC patient samples treated with OXA-based HAIC (Responders VS Non-responders). c Venn diagram showing top candidate genes involved in OXA resistance based on CRISPRa screen and RNA-seq analysis of HCC patient samples. d PRMT3 sgRNAs were enriched in OXA-treated HepG2 cells compared to vehicle-treated HepG2 cells. e Comparison of PRMT3 mRNA expres- sion (FKPM) in tumors from patients treated with OXA who were defined as responders and non-responders. f The mRNA and protein level of PRMT3 in PLC-

    Journal: Nature communications

    Article Title: PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer.

    doi: 10.1038/s41467-023-37542-5

    Figure Lengend Snippet: Fig. 1 | CRISPR activation screen and transcriptome analysis of patient samples identify PRMT3 as a candidate driver for OXA resistance in HCC. a Volcano plot reveals differential gRNAs targeting genes of genome-wide CRISPRa screen during OXA treatment. b Volcano plot shows the differentially expressed genes identified from RNA-seq analysis of HCC patient samples treated with OXA-based HAIC (Responders VS Non-responders). c Venn diagram showing top candidate genes involved in OXA resistance based on CRISPRa screen and RNA-seq analysis of HCC patient samples. d PRMT3 sgRNAs were enriched in OXA-treated HepG2 cells compared to vehicle-treated HepG2 cells. e Comparison of PRMT3 mRNA expres- sion (FKPM) in tumors from patients treated with OXA who were defined as responders and non-responders. f The mRNA and protein level of PRMT3 in PLC-

    Article Snippet: Genome-wide CRISPR activation screen In this study, the CRISPR-PoolTM SAM human library (Addgene, cat. no. 1000000074) was used to identify genes responsible for OXA resistance in HCC cells.

    Techniques: CRISPR, Activation Assay, Genome Wide, RNA Sequencing, Comparison